Roland Cy-5 Dual Trigger Cymbal Pad, 10In

£42.495
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Roland Cy-5 Dual Trigger Cymbal Pad, 10In

Roland Cy-5 Dual Trigger Cymbal Pad, 10In

RRP: £84.99
Price: £42.495
£42.495 FREE Shipping

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The main application for cyanine dyes is in biological labeling. Nevertheless, there is a wide literature on both their synthesis and uses, and cyanines are common in some CD and DVD media. The following protocol is an example of dye-protein conjugate purification by using a SepHadex G-25 column. where two quaternary nitrogens are joined by a polymethine chain. [5] Both nitrogens may each be independently part of a heteroaromatic moiety, such as pyrrole, imidazole, thiazole, pyridine, quinoline, indole, benzothiazole, etc. a b Ernst LA, Gupta RK, Mujumdar RB, Waggoner AS (Jan 1989). "Cyanine dye labeling reagents for sulfhydryl groups". Cytometry. 10 (1): 3–10. doi: 10.1002/cyto.990100103. PMID 2917472. For applications to biotechnology, special cyanine dyes are synthesized from 2, 3, 5 or 7-methine structures with reactive groups on either one or both of the nitrogen ends so that they can be chemically linked to either nucleic acids or protein molecules. Labeling is done for visualization and quantification purposes. Biological applications include comparative genomic hybridization and gene chips, which are used in transcriptomics, and various studies in proteomics such as RNA localization, [7] molecular interaction studies by fluorescence resonance energy transfer ( FRET) and fluorescent immunoassays.

For protein labeling, Cy3 and Cy5 dyes sometimes bear a succinimidyl group to react with amines, or a maleimide group to react with a sulfhydryl group of cysteine residues. Norman, D. G., Grainger, R. J., Uhrin, D. & Lilley, D. M. J. Location of cyanine-3 on double-stranded DNA: Importance for fluorescence resonance energy transfer studies. Biochemistry 39, 6317–6324 (2000). Cy3 fluoresces greenish yellow (~550 nm excitation, ~570nm emission), while Cy5 is fluorescent in the far-red region (~650 excitation, 670nm emission). [12] Cy3 can be detected by various fluorometers, imagers, and microscopes with standard filters for Tetramethylrhodamine (TRITC). Due to its high molar extinction coefficient, this dye is also easily detected by naked eye on electrophoresis gels, and in solution.Iris Dyes | Cyanine Technologies". Archived from the original on 2015-01-26 . Retrieved 2015-01-26. Umezawa K, Matsui A, Nakamura Y, Citterio D, Suzuki K (2009). "Bright, color-tunable fluorescent dyes in the Vis/NIR region: establishment of new "tailor-made" multicolor fluorophores based on borondipyrromethene". Chemistry: A European Journal. 15 (5): 1096–106. doi: 10.1002/chem.200801906. PMID 19117043. or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST

The additive effect of multiple Gs or Cs in a row is not immediately apparent, with GGGGG ranking #31 and #21 (0.71 and 0.64 relative intensity) and CCCCC ranking #1009 and #1021 (0.37 and 0.25 relative intensity) for Cy3 and Cy5, respectively. The top-ranking GAAAA motif identified in 5ʹ-Cy3 oligonucleotides here ranks #334 (0.58 relative fluorescence), further illustrating the significant differences between 5ʹ and 3ʹ labeling. commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying Sale, lease, license or other transfer of the material or any material derived or produced from it. Schneider, T. D. & Stephens, R. M. Sequence logos—a new way to display consensus sequences. Nucleic Acids Res. 18, 6097–6100 (1990).The reaction tubules were placed in a dark place and incubated gently at room temperature for 60 minutes at intervals.For 10-15 minutes, gently reverse the reaction tubules several times to fully mix the two reactants and raise the bar efficiency. Holz, K. et al. High-efficiency reverse (5’–>3’) synthesis of complex DNA microarrays. Sci. Rep. 8, 15099 (2018).



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