The genomic targets included in the CDS were identified by searching the published medical literature. The genomic targets were selected primarily through literature and database searches, including PubMed, MDPI, LitVar, and dbSNP. Information was gathered regarding SNP allelic frequencies. While many gene variants have been associated with autism, rare deleterious and pathogenic variants, such as those found in <1% of the population, were purposely not included. This exclusion of low-frequency and pathogenic variants was due to the idea that ASD can develop based on the combinatorial effects of multiple variants found in higher frequencies. So far, I can tell you that my bloating and gas flared up the first 2 days after taking inulin. I didn’t react at all to the oils. In search of the literature regarding regulators of degradation of SHANK3, a paper demonstrating that ERK2 is critical for the marking of SHANK3 for degradation was identified [ 25]. ERK2 phosphorylates SHANK3 to promote its ubiquitination, the “tagging” signal for degradation. The phosphorylation of SHANK3 causes the dissociation of SHANK3 from membranes, where it is degraded and, thus, cannot perform its scaffolding function. Inhibition of the ERK2 pathway increases SHANK3 abundance [ 25], and therefore inhibiting ERK2 has been proposed in the literature as a strategy to help to increase SHANK3 levels [ 25].

It’s organic and sourced from blue agave with no fillers or preservatives. I just mix it in about a cup of water and drink it, but you can also add it to applesauce, oatmeal, yogurt, whatever. I definitely need to update this post with my latest experience on the Nemechek Protocol. It’s been such a blessing! I’m just now on full doses of everything (about 5 months into it), and I’m finally seeing a turning point. It’s really hard to explain, but I feel like my brain fog is dissipating. I’m thinking more clearly, I’m not having as hard a time finding words, anxiety is way better, etc.Around Day 5 of taking the antibiotics for SIBO, I noticed a significant decrease in bloating. Now, I’m about 3 weeks post-Rifaximin, and I have little to no bloating anymore. It’s all a guessing game, but I suspect some sort of food poisoning with that salad ( I’m looking at you, romaine!). The National Institute of Health and Care Excellence (NICE) will only recommend something if there is good evidence that it benefits autistic people. Information on SHANK3 was curated from the peer-reviewed scientific literature to determine the function of both the gene and the effect the particular single nucleotide polymorphism (SNP) found in these cases creates.

The treatment involves a two-step process that aims to rebalance intestinal bacteria and omega fatty acids, which in turn allows the child's brain to begin to heal and function properly. The basics of the approach consist of cooking with extra-virgin olive oil and introducing inulin and omega-3 fatty acid supplements into a child's daily regimen, and the book lays out the exact steps and appropriate doses, based on age. Around week 3, I started getting nauseous after taking the supplements. I’m not really sure what’s causing that, but I’ve asked around in the Nemechek Protocol community, and it seems to be par for the course and something that I need to just push through. This variant is suggested to increase TCN1 expression, which is associated with lower vitamin B12 levels. This is due to TCN1 carrying 80–90% of serum B12, with the rest being carried by TCN2 to the brain and other organs. Upregulation of TCN1 lowers the B12 available for TCN2 [ 59]. There is a very helpful Facebook group that you can join – https://www.facebook.com/groups/TheAdultNemechekSupportGroup/ This variant is associated with lower enzymatic activity, specificity for substrate, and thermal stability. This decreases the ability to conjugate reduced glutathione with toxins for removal [ 61].Dr. Nemechek believes that probiotics have the potential to increase the numbers of all bacteria, as opposed to just the “good” guys. While on this protocol, he advises to stop probiotic supplements, but fermented foods such as sauerkraut and kefir are fine. So what he is saying is that you can add to your diet, Extra Virgin Olive Oil, Omega 3 and Prebiotic - all of these things are natural. EVOO is medically proven to lower inflammation and good for general health, Omega 3 is proven for brain health and is naturally occuring in salmon - i do not like fish but I take Omega 3 to help my brain and prebiotics which is made from chicory. He is not asking you to take any of these things over the normal RDA.