Postie (Series 1), a postman who always tries (but fails) to deliver the post on time. At just 3 sketches, Postie is the least frequent character in the entire series. He doesn't speak. (In Series 1, Episode 14, he did cheer and laugh.) Will Barrow (Series 3–4), a gardener whose gardening experience is always ruined by the animals residing in his garden. He doesn't speak. Milkshake Jake (Series 1), a sundae bar worker who has a messy line in milkshakes and always gets distracted by the professions of his customers.

Series 1 was filmed from March 2009 to April 2009, Series 2 was filmed from July 2010 to August 2010, Series 3 was filmed from May 2013 to June 2013, Series 4 was filmed from July 2014 to August 2014, Series 5 was filmed from March 2018 to May 2018 and GiggleQuiz was filmed between June 2019 to August 2019. Will Singalot (Series 3-present), a minstrel and one of Robin Hood's Merry Men who infuriates Robin Hood with his nonstop singing and always spoils Robin's efforts to impress Maid Marian.King Flannel (Gigglebox Pilot, Series 1–3), a naughty, childish, old monarch who needs to be kept in line by his beady-eyed and long-suffering butler for several reasons. Between series 1 and 2 He only spoke in gibberish sounds, but by series 3, his dialogue became a lot more understandable, he also appeared in the original "Gigglebox" pilot.

Keith Fitt (Series 2-present, GiggleQuiz), a Geordie sports instructor whose "20–year" experience hasn't gained him any skills. Keith is one of the team captains in GiggleQuiz.

Arthur Sleep (Showreel, Series 1-present, GiggleQuiz), a newsreader of a Northern origin who is a bad joke-teller, is always falling asleep, and is also the host of GiggleQuiz. Additionally, he appeared in the "Justin Time" showreel. Wild Wez (Series 5-present), a cowboy who always focuses on how to clean up his barn, but his barnyard animals always mess it up again. He doesn't speak. The TV series was starred and created by Justin Fletcher, who plays the lead character in all of the comedy sketches and also wrote a few sketches in its first series. Some of the sketches are filmed in the studio, while other sketches are filmed outside; for example, one is filmed at Portmeirion, used for the fictional town of Wiggyville where Captain Adorable's sketches are set from Series 1 to 2. The show is interspersed with a selection of children called the Gigglekids, who tell jokes to Justin himself, in their own section called 'Giggle Box'. Dina Lady (Series 1–2, 4-present, GiggleQuiz), a homely cook who takes the names of dishes to their very literal meanings and whose assistant is Tommy Tummy. In Series 4 Dina became the host of a Great British Bake-off-esque show, with Tommy as her co-host. In Series 5 Dina became the delivery driver for a catering company. Anna Conda (Showreel, Series 1), a reptile expert who makes deliberately cheesy jokes based on reptiles. She also appeared in the "Justin Time" Showreel along with Arthur Sleep.