HG Car Dashboard Cleaner, For a Shiny Interior, Cleans & Restores Shine, Leather & Plastic Safe, Intensifies the Colour, Protects from Dust, Fresh Fragrance Polish – 400ml Spray (536040106)

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HG Car Dashboard Cleaner, For a Shiny Interior, Cleans & Restores Shine, Leather & Plastic Safe, Intensifies the Colour, Protects from Dust, Fresh Fragrance Polish – 400ml Spray (536040106)

HG Car Dashboard Cleaner, For a Shiny Interior, Cleans & Restores Shine, Leather & Plastic Safe, Intensifies the Colour, Protects from Dust, Fresh Fragrance Polish – 400ml Spray (536040106)

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It will have your employee’s name as held in the Payroll system, associated with this 'HG' account: Manual entry code method HG car dashboard cleaner makes the interior of the car look like new. This dash cleaner cleans and protects synthetic materials, plastic, rubber, vinyl and leather. Also, the car dash cleaner intensifies the colour and restores the shine of the polished surface. The HG dashboard spray also leaves a wonderfully fresh fragrance after the treatment, but not a detectable greasy coating. How do you use HG car dashboard cleaner? A conserved region of merA gene encoding bacterial mercuric reductase enzyme was detected using the above isolated genomic DNA ( Joshi etal., 2021). The PCR reactions were carried out using the primer set F1merA (5’-TCGTGATGTTCGACCGCT-3’) and F2merA (5’-TACTCCCGCCGTTTCCAAT-3’) containing 1 U/μl Taq polymerase (Sigma-Aldrich), 1 × Enzyme buffer, 200 μM of each dNTP (Sigma-Aldrich), 1.25 mM MgCl 2, and 0.5 μM of each primer and 50 ng template DNA in a thermal cycler (Applied Biosystems). PCR reaction was carried out under the following amplification conditions: pre-denaturation step at 94°C for 2 min followed by 30 cycles of 94°C for 1 min, 52°C for 1 min and an extension step at 72°C for 1 min and final extension at 72°C for 7 min as described by Dash and Das, (2014). For the isolates that showed negative PCR reactions with F1merA and F2merA primer set, a re-attempt has been made for amplifying the merA gene by gradient PCR method (annealing at 54 ± 5°C) and by using another set of primer A1F (5′-ACCATCGGCGGCACCTGCGT-3’) and A5R (5′-ACCATCGTCAGGTAGGGGACCAA3-′) as described by De etal. (2008). Amplification of the merA gene was confirmed by visualizing it under the Gel Doc system (BioRad). Confirmation of Hg 2+ to Hg 0 reduction by the isolates Based on the results obtained by the x-ray film method, ICP-MS analysis has been carried out to confirm the Hg removal potential of the isolates in the presence of 50 mg/L of Hg 2+. The ICP-MS outcomes showed significant changes in the level of Hg depletion. Among all, the marine culture NIOT-EQR_J251 possessed the highest potential (70.62%) to volatilize Hg. NIOT-EQR_J258 showed the least removal of Hg (17.48%), whereas NIOT-EQR_J7 and NIOT-EQR_J248 were capable to volatilize 29.18% and 52.17% of Hg 2+ under similar conditions, respectively. ( Figure4B). NIOT-EQR_J251 could volatilize 32.54 mg of the initial inoculum of 46.07 mg of HgCl 2, whereas NIOT-EQR_J258 removes 8.03 mg of the initial supplement of 45.74 mg of Hg 2+. In presence of 50 mg/L of Hg 2+, NIOT-EQR_J7 and NIOT-EQR_J248 dominantly developed the fog on the x-ray film, whereas, in the ICP-MS analysis, NIOT-EQR_J251 showed the highest Hg removal as compared to the other isolates. This suggests that volatilization was not solely responsible for removing the Hg 2+ from the culture media and the contribution of different processes like bioaccumulation and bio-adsorption may be involved. Mercury (Hg) pollution poses a global threat to human and environmental health due to its noxiousness, mobility, and lengthy residence duration in the atmosphere ( Raphael etal., 2011). Toxic metals rapidly accumulate in the food chain, impacting higher trophic levels, hence these are of principal concern nowadays ( Raphael etal., 2011). According to recent research, oceanic release and biomass burning (organic compounds) account for the majority of worldwide Hg emissions, with anthropogenic activities accounting for the remaining significant percentage ( Pirrone etal., 2010; Nelson etal., 2012; Serrano etal., 2013). Hg exists in elemental, inorganic, and organic forms in both land and water systems, depending on oxidation-reduction conditions. In the atmosphere, Hg with valence +2 is more extensively spread ( Wang etal., 2004). In the biogeochemical cycle, a considerable part of Hg accumulates in seas and oceans by its atmospheric deposition ( Bindler, 2003; Wang etal., 2004). The effects of human-induced sources on the Hg contents and its forms are extensively larger in the photic layers of oceans ( Strode etal., 2007).

The GC-MS study was included to get insight into the metabolic changes in the MRB isolates under Hg stress. It revealed that the metabolic profiles of MRB isolates are diverse with different biological properties. GC-MS metabolic profiles revealed that the number of compounds increased in the presence of Hg compared to the control isolates, where some compounds were found to be different in Hg-treated samples. A total of 50 metabolites were identified in the absence of Hg, whereas with 50 mg/L of Hg, 64 compounds were identified. The minimum metabolites (6) were identified in NIOT-EQR_J258 without Hg, whereas the maximum metabolites (22) was identified in NIOT-EQR_J251 with Hg 2+. The NIOT-EQR_J7 metabolic profile was almost the same in both control and Hg-treated samples. A total of 14 metabolites were present in the control sample, whereas 15 metabolites were identified with Hg. In the case of NIOT-EQR_J248, NIOT-EQR_J251, and NIOT-EQR_J258, a total of 10, 20, and 6 compounds were identified in the control sample, whereas 16, 22, and 11 metabolites were present with Hg, correspondingly. According to a study by Ozemek et al., healthy diets like DASH dietary patterns have been shown to decrease SBP by 11 mm Hg in hypertensive individuals and by 3 mm Hg in normotensive individuals. Ideally, the goal is to reduce sodium to <1500 mg/dl, but it is good to aim for at least a 1000 mg/day reduction, which has been shown to reduce SBP approximately by 5/6 mm Hg in hypertensive individuals and by 2/3 mm Hg in normotensive individuals [ 11]. Furthermore, Ozemek et al. revealed in their study that increasing dietary potassium intake with a goal of 3500-5000 mg/day has been shown to reduce SBP by −4/5 mm Hg in hypertensive individuals and by −2 mm Hg in normotensive individuals [ 11].

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In context to these four isolates, the shift in wavenumbers, changes in peak height, and the appearance/disappearance of new peaks in the presence of Hg 2+; suggest alterations in functional groups (especially -SH group), which might play an important role in the Hg detoxification. SEM analysis of Hg treated and untreated isolates The Mediterranean diet (Med Diet) has also helped lower BP. As per the Maine-Syracuse Longitudinal Study conducted in the United States in 2020 by a group of researchers who followed 851 US older adults, for every one unit increase in the Med Diet score in participants, it was found that there was a corresponding reduction of 0.69 units in SBP, a reduction of 0.33 in DBP, and a reduction of 0.45 on mean arterial pressure (MAP) [ 32]. Although this seems to be small, this change can have a noteworthy effect at the level of the population; that is, a decrease of 2 mm Hg in SBP can lead to a decrease of 10% when it comes to the population [ 32]. According to the observational studies conducted in Mediterranean countries, higher adherence to a Med Diet is associated with a decreased risk of cardiovascular disease, overall mortality as well as neoplastic disease [ 33, 34]. The Med Diet consists of higher consumption of extra virgin olive oil, vegetables, fruits, whole grains, nuts, cereals, as well as seeds; moderate consumption of fish, poultry, red wine, and dairy; and lower consumption of processed foods and red meat [ 35]. The selected isolates were further analyzed by subjecting them to 16S rDNA sequencing. In brief, DNA was isolated and the polymerase chain reaction (PCR) amplification was executed with the universal 16S rDNA primers of 27F (5’-AGAGTTTGATCMTGGCTCAG-3’) and 1495R (5’-GGHTACCTTGTTACGACTT-3’) as described by Dash and Das (2014). The amplified PCR products were sequenced and submitted to NCBI, as described by Kumar etal. (2018). The detailed procedure is elaborated in the SI. Genotyping of merA gene

Patients can find it increasingly difficult to maintain long-term dietary changes like low caloric intake, low salt intake, and limiting processed foods, which in turn may lead to a shift towards preintervention practices in some individuals, for example, increased calorie intake [ 28]. This implies the need for interventions to focus on ways to help patients maintain healthy dietary patterns [ 28]. Physicians play a key role in encouraging and helping patients achieve smoking cessation [ 56]. HTN has also been associated with second-hand smoke. According to research done by Bernabe-Ortiz et al. on 897 individuals in Peru in 2021 to assess the association of second-hand smoke with HTN and cardiovascular risk, 15% of adults reported second-hand smoke overall, and this emphasizes the necessity to keep places smoke-free to reduce the risk of cardiovascular disease [ 57]. The upholstery suffers most. We have HG car upholstery cleaner. Or take a look at our tips for cleaning fabric car upholstery. For leather seats, we have HG deep cleaner for leather. Hg tolerance of CMRBs was calculated by the broth dilution method ( Konopka and Zakharova, 1999; Santos-Gandelman etal., 2014) with some modifications. CMRB cultures grown in Zobell Marine Broth medium (ZMB, Himedia, Mumbai) without Hg supplementation were used as a positive control, whereas ZMB treated with Hg (without bacteria) was used as a negative control in this study. To ensure the uniform bioavailability of Hg to the bacteria, uniform culture conditions were maintained across different experimental treatments. Based on the growth behavior of cultures in the presence of a higher concentration of HgCl 2, the selected isolates were classified into two different categories i.e. MRB and moderate MRB (MMRB). The detailed procedure is elaborated in the SI. To understand the effects of different Hg concentrations on bacterial growth, two qualitative analyses were carried out, i.e. monitoring the optical density (OD) at 600 nm and dry biomass. 16S rDNA based identification The datasets presented in this study can be found in online repositories. The names of the repository/repositories and accession number(s) can be found in the article/ Supplementary Material. Author contributions

The modification in functional groups present in the culture pellets was identified by measuring the spectra in the range of 400 to 4000 cm -1 using Fourier transform infrared (FT-IR) spectroscopy (IR Affinity-I spectrometer, Shimadzu, Japan), as described by Joshi etal. (2021). In brief, 48 h grown cultures in ZMB medium without Hg supplementation were used as a control, whereas cultures with 50 mg/L of Hg supplementation were used as Hg treated. The mixtures (lyophilized cells and 2% KBr) were fixed in the FT-IR spectrometer after compressing them into translucent sample discs, followed by analyzing in ATR-FT-IR mode by following the manufacturer’s protocol. Scanning Electron Microscopy (SEM) analysis of MRB Our preliminary study suggests that the detoxification of Hg 2+ is the immediate result of the GST and merA function as shown in Figure S6. The existence of the glutathione reductase gene in some bacterial mer operons also supports the role of LMW thiols in Hg 2+ detoxification ( Norambuena etal., 2018). The E. coli, together with the integrated merA - GST gene, was able to survive in the high Hg stress environment and transform Hg 2+ to Hg 0 ( Cursino etal., 2000). It is correlated that IOTG may act as Hg(II)-buffering agents and subsequently, Hg 2+ is reduced by merA. In the case of merA, mercuric ion was uptaken by the active process i.e. mer mediated transport (merP and merT) and converted to Hg 0 form by mercuric reductase. On the other hand, the Hg 2+ that was uptaken through the passive process could subsequently be inactivated by GST or conjugated to merA. Thus, an alternative method is proposed via which Hg resistance level may be augmented in bacteria: the sequestering of Hg given a protein-ligand interaction may lead to an improved way for the volatilization process. Conclusions One of the quintessential steps to manage HTN is lifestyle modifications like exercise, weight loss, dietary interventions, a low-sodium diet, limiting alcohol consumption, smoking cessation, and stress management to help control BP. The treatment of HTN is based on specific characteristics like stage of disease, compliance, and comorbid conditions. For proper management of HTN, we may need to include pharmacological and non-pharmacological interventions [ 5]. Despite advancements in medicine and treatment options, the global burden of HTN has been increasing due to the advancing age of the population and an increasing prevalence of obesity. HTN is an insidious disease that, if not treated promptly, predisposes us to cardiovascular complications and various other complications [ 6]. Increased physical activity, limiting salt intake, minimizing alcohol consumption, smoking cessation, and stress management together support the management of patients with HTN and as preventive measures in the general population [ 7]. However, it is important to note that lifestyle modification is a process that requires patients to adhere continuously [ 5]. Based on phenotype characteristics, a total of 162 bacterial colonies grown on the ZMA media supplemented with 10 mg/L of Hg as HgCl 2 were selected for further analysis. In the presence of 25 mg/L of Hg 2+, only 63 isolates out of 162 showed resistance. Further, these 63 isolates were characterized based on their growth pattern in the presence of more than 25 mg/L i.e. 50, 75, and 100 mg/L of Hg 2+ concentration. Among the 63 isolates, 21 isolates grew in the presence of 50 mg/L, followed by 9 and 4 isolates at 75 and 100 mg/L of HgCl 2, respectively. The growth pattern of 4 isolates in the presence of HgCl 2 is shown in Figure S1.

A simplified X-ray film method was adopted to look into the Hg reduction by bacterial volatilization in the presence of 50 and 100 mg/L of Hg as HgCl 2 ( Nakamura and Nakahara, 1988; Joshi etal., 2021). Briefly, the bacterial cells were collected by centrifugation at 5,000 rpm for 10 min. and washed with 0.07 M phosphate buffer (0.5 mM EDTA, 0.2 mM magnesium acetate, 5 mM sodium thioglycolate; pH 7.0) and transferred to the microplate. The cells were suspended into 50 µL of 0.07 M phosphate buffer containing 50 and 100 mg/L of Hg as HgCl 2 in a microplate. The phosphate buffers (pH 7.0) with 50 and 100 mg/L of HgCl 2 (without bacterial cells) were used as a negative control. The plate was covered with X-ray film and incubated in the dark for 2 h at 35°C. Determination of Hg(II) depletion potential by isolatesEnter [email protected] as the Account name and then enter or paste the manual entry code in from above as the 'Secret key'. Assorted studies have shown that IF lowers BP. Harvie et al., in their study involving 107 overweight or obese premenopausal women, showed that IF for six months helped lower SBP (p= 0.99) and DBP (p= 0.84) [ 22]. Varady et al., in their study performed for 12 weeks with IF involving 15 overweight individuals (five males, 10 females with a BMI of 20-29.9 kg/m 2 showed that IF helped lower BP with a p-value of 0.51 [ 23]. A study by Bhutani et al. involving 83 obese individuals (three males and 80 females) with a BMI of 30-39.9 kg/m 2revealed that 12 weeks of IF helped lower SBP (p = 0.254) and DBP (p = 0.570) [ 24]. In a study by Eshghinia et al., 15 overweight or obese women with a BMI ≥25 kg/m 2 who followed IF for eight weeks showed a lowering of SBP (p<0.001) and DBP (p<0.05) [ 25]. Teng et al., in their 12 weeks IF study with 28 Malay men with a BMI of 23-29.9 kg/m 2,showed a lowering in SBP (p<0.05) and DBP (p<0.05) [ 26]. Erdem et al., in their study involving 60 participants from the Cappadocia cohort with pre-HTN and HTN with SBP of 120-139 and more than or equal to 140 mm Hg, DBP of 80-80 and more than or equal to 90 mm Hg, revealed that IF helps lower SBP (p<0.001) and DBP (p<0.039) [ 27]. Three experimental replicates were determined for each analysis and the statistical significance of the results was analyzed by one way ANOVA test. All Statistical analyses were carried out using SPSS (version 17) and GraphPad Prism 7 software. Differences were considered significant at p< 0.05 and values are reported as mean ± standard deviation (SD). Results and discussion Although weight loss plays a significant role in lowering BP, it may be challenging to stay constantly motivated for long-term results [ 28]. Developing strategies to identify individuals who are unable to maintain lifestyle changes may be crucial to help them stay motivated to achieve weight management goals [ 28]. Furthermore, in recent times, an effective tool to promote the maintenance of healthy lifestyle changes like weight loss can be the use of mobile technology and personal digital devices, especially when individuals no longer have the availability of support and accountability through active interventions [ 29]. In hypertensive individuals with normal weight, other interventions like the DASH diet help lower BP [ 28]. The DASH diet has been funded by the National Institute of Health (NIH) in various research projects to know whether specific dietary interventions helped treat HTN [ 30]. The subjects included in the study have been told to follow only dietary interventions and no other lifestyle modifications to avoid any confounding [ 30]. It was found in both hypertensive and normotensive individuals that dietary intervention alone helped reduce SBP by 6 to 11 mm Hg [ 30]. Since these results, the DASH diet, along with lifestyle modifications, has been advised as the first line of pharmacologic therapy in some instances. Several other clinical trials have shown that the DASH diet helps lower BP, cholesterol, and saturated fats as well as there is evidence that it lowers the risk of adverse cardiac events, type 2 diabetes, stroke, and obesity [ 30]. Therefore, it is crucial for clinicians, nurses, and pharmacists to educate patients about the benefits of the DASH diet [ 30].



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