Master Series Black Silicone Bone Gag

Product Information

Using a ball gag, lip gag or even a ring gag is pretty safe all things considered. There is not much harm they can do, but for beginners, there are still some things I would like to point out.

The noteworthy exception to the following steps in GAG biosynthesis is hyaluronic acid (HA). Instead of undergoing modification and sulfation in the Golgi apparatus, the HA precursor sugars UDP-glucuronic acid and UDP- N-acetylglucosamine are transported from the cytoplasm to the plasma membrane for further processing without sulfation, which leads to the production of HA. [4] Clark RD, Smith JG Jr., Davidson EA (1965) Hexosamine and acid glycosaminoglycans in human teeth. Biochim Biophys Acta 101: 267–272. pmid:4222028 Embery G, Hall R, Waddington R, Septier D, Goldberg M (2001) Proteoglycans in dentinogenesis. Crit Rev Oral Biol Med 12: 331–349. pmid:11603505

Author Contributions

Citation: Coulson-Thomas YM, Coulson-Thomas VJ, Norton AL, Gesteira TF, Cavalheiro RP, Meneghetti MCZ, et al. (2015) The Identification of Proteoglycans and Glycosaminoglycans in Archaeological Human Bones and Teeth. PLoS ONE 10(6): Waddington RJ, Roberts HC, Sugars RV, Schonherr E (2003) Differential roles for small leucine-rich proteoglycans in bone formation. Eur Cell Mater 6: 12–21; discussion 21. pmid:14562268 Salbach J, Rachner TD, Rauner M, Hempel U, Anderegg U, et al. (2012) Regenerative potential of glycosaminoglycans for skin and bone. J Mol Med (Berl) 90: 625–635.

Lip gag– a lip gag is a bit different from your standard ball gag, and it is rarer to see in movies but it has its perks. The lip gag usually goes on your lips, as the name says, but it has a hole and your teeth will be behind it meaning your mouth will stay open. This leads to possibly some of the best blowjobs you will ever give/receive or letting your partner explore the inside of your mouth with his fingers. Plus, various designs make them catchy and good-looking. The downside is that they don’t prevent loud noises as much as a ball gag would. PGs are macromolecules comprised of one or more GAG chains covalently bound to a protein core [ 11]. GAGs are linear polysaccharides composed of repeating disaccharide units which consist of a hexosamine and either hexuronic acid or galactose units, and may be sulfated in various positions. The strategic variability in sulfate substitution results in considerable sequence heterogeneity which conveys the plethora of biological roles GAGs play in cell-cell and cell-matrix interactions. GAGs are classified into six groups: chondroitin 4- and 6-sulfate (C4S, C6S), keratan sulfate (KS), hyaluronic acid (HA), dermatan sulfate (DS), heparin and heparan sulfate (HS).Jewelry and hair – piercings can make all the difference in what sort of gags and straps you want to put on yourself or your partner. Keep that in mind when buying stuff. Also, longer hair can lead to pulling and pain, so be sure to tie it up and adjust your accessories accordingly.

Nikitovic D, Aggelidakis J, Young MF, Iozzo RV, Karamanos NK, et al. (2012) The biology of small leucine-rich proteoglycans in bone pathophysiology. J Biol Chem 287: 33926–33933. pmid:22879588 GAGs (60 μg) extracted from archaeological human bone samples were subjected to β-elimination to ensure that the GAGs (other than N-linked KS chains) had been released from the protein core. Briefly, the GAG pellet was suspended in 200 μL sodium borohydride (0.0378 g Na(BH 4) in 1 mL NaOH 0.1 M) and kept overnight at room temperature. The solution was then neutralized using 10% acetic acid (approximately 110 μL). A volume of 500 μL of MilliQ water was added and the solution transferred to 1 kDa dialysis tubes (GE Healthcare Bio-Sciences Corp., NJ, USA), and dialyzed at room temperature for 4 hours in distilled water. The solution was then lyophilized. The GAG pellet was suspended in 60 μL MilliQ water and the sample divided in three, boiled for 15 min to inactivate any residual maxatase, and lyophilized. The first sample was suspended in 2.5 μL heparin lyase II (1 U/mL, Sigma-Aldrich, St. Louis, MO) and 7.5 μL ethylenediamine acetate buffer 0.05 M (pH 7.0), the second sample in 20 μL Flavobacterium heparinum extract and the third sample in 20 μL MilliQ water. The samples were incubated overnight at 30°C, lyophilized, suspended in 5 μL MilliQ water, and analyzed by agarose gel electrophoresis as described above. The crude extract of Flavobacterium heparinum grown in the presence of heparin degrades heparin and HS [ 35]. Heparitinases I and II, and a heparin lyase have been identified in the crude extract of Flavobacterium heparinum [ 36, 37]. The crude extract of Flavobacterium heparinum also degrades HA, C4S and C6S [ 38]. In addition, the crude extract of Flavobacterium heparinum grown in the presence of CS degrades DS [ 39, 40].

Shipping & Returns

Young MF (2003) Bone matrix proteins: their function, regulation, and relationship to osteoporosis. Osteoporos Int 14 Suppl 3: S35–42. pmid:12730768 The 4.5-inch circumference rubber ball is the perfect size for my bit-smaller mouth. It has D-shaped rings on both sizes that make my mouth look extra slutty when I’m all tied up with the head strap that is fully adjustable for the perfect fit. The ball gag I’m all hyped up about can be found here. Story Of The Time I Used This Ball Gag Martins JR, Passerotti CC, Maciel RM, Sampaio LO, Dietrich CP, et al. (2003) Practical determination of hyaluronan by a new noncompetitive fluorescence-based assay on serum of normal and cirrhotic patients. Anal Biochem 319: 65–72. pmid:12842108 Given the vital role SLRPs and GAGs play in bone morphogenesis and homeostasis, alterations in their expression profile or their loss due to unregulated proteolysis leads to a plethora of diseases. The expression of PGs and GAGs is altered in various bone related diseases, including osteoarthritis of the temporomandibular joint [ 31], osteochondromas and peripheral chondrosarcomas [ 32], and due to this, these molecules can be used as biological markers.