To further increase the screening throughput, emphasis was put at developing a screening method based on fluorescence-activated cell sorting (FACS). Employing FACS, one can routinely sort > 107 clones or cells per hour. Different approaches such as coupling FACS with oxygen sensitive dyes have been evaluated for technical feasibility. Finally, a whole cell ultra-high throughput screening platform for P450 monooxygenases based on flow cytometry was established that is based on the dealkylation of a coumarin derivative. This platform is not only restricted to P450 monooxygenases activity, but can also find applications in metagen Starting from crude sample isolation, followed by an HPLC separation based on MS analysis and product isolation via a solid phase extraction unit, the sample was analyzed using a NMR spectrometer for structural analysis. Hence, concentrated fermentation samples were re-dissolved in a mixture of ACN/MeOH and HPLC analysis was conducted. By using an XB-C18 column and an ion trap mass spectrometer it was possible to identify the three main fermentation products. After identification of the desired hydroxylated metabolites via mass spectrometry, structural analysis via NMR spectroscopy was performed to distinguish between different hydroxylation positions. The major hydroxylated product that we were able to isolate and identify, based on HPLC/MS-SPE/NMR analysis and previously published spectral data were 2,17-dihydroxy-(2β,17β)-androst-4-en-3-one. Additional 2-D experiments (COSY and HSQC) and the change of solvent completed the analysis.

Zeng Y, Pu X, Yang J, Du J, Yang X, Li X, Li L, Zhou Y, Yang T. Preventive and Therapeutic Role of Functional Ingredients of Barley Grass for Chronic Diseases in Human Beings. Oxid Med Cell Longev. 2018 [cited 2022 January 20];3232080. DOI: 10.1155/2018/3232080. Acetyl L-Carnitine HCl, Garcinia Cambogia Fruit Extract (60% Hydroxycitric Acid), Conjugated Linoleic Acid (CLA), Grapefruit Seed Extract 4:1, Raspberry Ketones (From Raspberry Fruit Extract), Mangifera Indica Seed Extract, Bitter Orange Fruit Extract, Green Coffee Bean Extract (50% Chlorogenic Acid), Olive Leaf Extract (10% Oleuropein), Guggul Extract Powder, Chromium Picolinate Kiwi Strawberry: Natural Flavor, Citric Acid, Sucralose, Silicon Dioxide, Calcium Silicate, Spirulina Extract (Color), Turmeric (Color) The phosphate-based screening assay has been described in two scientific publications and has already helped to identify interesting enzymes, see below. OxyGreens not only contains a green superfood complex but also antioxidants, prebiotic fiber and an array of other wellness ingredients in a convenient 1-scoop serving. The best part is all these benefits are delivered with an amazing taste.We created OxyGreens to be the MOST DELICIOUS natural, super greens on the market, enhanced with alkalizing forest berries and wholefood prebiotic fiber. Antioxidants also help combat free radicals to promote glowing, healthy skin and boost immunity. Prebiotic fibre contributes to healthy digestion and staying regular. Our newest flavors are Passionfruit and Mango, both of which have been very popular. All the OxyShred flavors are delicious, but it really depends on your taste preference. Our daily greens powder contains a prebiotic fiber complex which includes; ​​Chicory Root (Inulin), Green Kiwi Fruit, Green Banana, and Larch. These superfoods help to nourish the good gut bacteria (microbiome) and promote an overall healthy digestive system which improves nutrient absorption in the gut lining, promotes bowel regularity and reduces stomach bloating. Support Liver detoxification: The leading ingredients of the Green superfood complex are Barley Grass, Broccoli, Kale, and Spinach. These powerful greens are rich in magnesium and vitamins A, K, E and C, which act as antioxidants and help support a healthy immune system. Antioxidants help the body remove unwanted toxins that can build up as a result of natural processes and environmental stressors, reducing the risk of illness and disease.

Yes! OxyShred helps put you in a positive and focused mindframe to conquer your workout. Whether you are a beginner or a seasoned pre-workout consumer, you’ll love the natural positive effects of OxyShred during your workout. Unlike some other products on the market, you shouldn’t get jitters from OxyShred if used as directed as it’s not over-caffeinated.This is a RERA registered project with RERA ID P52100011154 and fulfils all conditions placed by the state regulatory body. Take the hard work out of reaching your micronutrient requirements, for an incredible taste of the best greens supplement you simply won’t resist! With a range of delicious Oxygreens flavours, there is one for every taste. Which is the best Oxygreens flavour for you? Easy, simply try them all. As suggested in the Directions, Acetyl L-Carnitine is best taken with OxyShred to maximize its effectiveness. Boost Immunity & Vitality: Additional greens including; Celery, Spirulina, Chlorella and Kelp have been added into this green superfood powder. They are rich in vitamins, minerals, iron and chlorophyll to help maintain a healthy digestive function and support overall wellbeing. Studies suggest Spirulina may also help to maintain a healthy immune system.