Simon Pieman (Christmas special, Series 2), a pie deliveryman whose deliveries are done on wheels and whose pies always end up in his face. The first series was broadcast in September 2009, comprising 25 13-minute episodes and 20 characters each. The first episode premiered on 7 September 2009. A Christmas special was broadcast on 15 December 2010. The second series comprised 15 episodes being broadcast in January 2011 and saw several characters disappear to make way for five new ones, replacing them. The spinoff quiz show GiggleQuiz came after Gigglebiz, premiering on 28 October 2019 and later in December 2020.

Rapids Johnson (Gigglebox Pilot, Series 1-present, GiggleQuiz), a wildlife explorer who's always on the hunt for elusive wildlife (usually bears) but never actually gets to spot them. In the Gigglebox pilot, Rapids was called Jungle Jimbo and was Australian, parodying Steve Irwin. Rapids also appeared in the Justin's House episode, Teddy Bears' Picnic. Enrico Paso Doble (Series 3-present), a dancer who isn't very good at dancing and gets distracted during his routines. He is played by Justin and doesn’t speak. (He did apologise in Series 3 Episode 1 and does say "Arrivederci!" in the Series 4 and 5 credits only.) Dan Step (Series 3-present, GiggleQuiz), a tap dancer who often gets confused. He doesn't speak. The only GiggleQuiz episode he appeared in was The Lost Pirate's Treasure.Professor Muddles (Series 1-present, GiggleQuiz), an eccentric scientist who often gets words mixed up and whose experiments never go to plan. Gigglebiz is a British children's comedy television programme (described as a 'live-action comedy sketch show' [5] by the BBC) consisting of five series that have been broadcast on CBeebies, [4] the BBC's younger children's channel, starting in 2009.

Humphrey (Humphrey the Painter Pilot, Series 1, 3-present), a silent man who is irrepressible and inquisitive. His trusty dog companion and best friend is Woody the dog. Like with Captain Adorable, Humphrey originally appeared in his own standalone pilot (where he was a painter) before being added to Gigglebiz. (The only time Humphrey spoke was in the outtakes) Will Barrow (Series 3–4), a gardener whose gardening experience is always ruined by the animals residing in his garden. He doesn't speak. Milkshake Jake (Series 1), a sundae bar worker who has a messy line in milkshakes and always gets distracted by the professions of his customers.

Series 1, Episode 15

Lady Chatalot: (Julie Alanah Brighten) King Flannel's friend. Flannel is always jealous of her and gets more than Flannel does. (Recurring in Series 1 to 2, regular in Series 3) Chip Monk (Gigglebox Pilot, Series 1), an eccentric pet shop worker who tries his best not to have to sell the animals in his shop to his customers. He also appeared in the original "Gigglebox" pilot. The Burrito Brothers (Gigglebox Pilot, Series 1–4), a trio of strongmen who haven't seen good days. The older two are played by Karl Magee and Gary Cross. The youngest brother, played by Justin, always unintentionally ruins their acts in some way. They don't speak, although they do make vocal sounds. They also appeared in the original "Gigglebox" pilot. (In the Gigglebox Promo, the third Burrito Brother said, "Watch the Gigglebox, on CBee-Bee-Beebies.") Series 1 was filmed from March 2009 to April 2009, Series 2 was filmed from July 2010 to August 2010, Series 3 was filmed from May 2013 to June 2013, Series 4 was filmed from July 2014 to August 2014, Series 5 was filmed from March 2018 to May 2018 and GiggleQuiz was filmed between June 2019 to August 2019.

When the showdown starts, a timer usually appears in the bottom left corner of the screen, however it didn't appear in "A Giggle Quizmas".

Series 2, Episode 15

The TV series was starred and created by Justin Fletcher, who plays the lead character in all of the comedy sketches and also wrote a few sketches in its first series. Some of the sketches are filmed in the studio, while other sketches are filmed outside; for example, one is filmed at Portmeirion, used for the fictional town of Wiggyville where Captain Adorable's sketches are set from Series 1 to 2. The show is interspersed with a selection of children called the Gigglekids, who tell jokes to Justin himself, in their own section called 'Giggle Box'. Doreen: Arthur Sleep's coworker. Her arm only appears. (Infrequent in Series 1 and 2, regular in Series 3 - present) Dina Lady (Series 1–2, 4-present, GiggleQuiz), a homely cook who takes the names of dishes to their very literal meanings and whose assistant is Tommy Tummy. In Series 4 Dina became the host of a Great British Bake-off-esque show, with Tommy as her co-host. In Series 5 Dina became the delivery driver for a catering company.